User Manual Corporate Headquarters Invitrogen Corporation Faraday Avenue Carlsbad, CA T: 1 7F: 1 7E: tech_support@www.doorway.ru of a gene of interest into a vector for entry into the Gateway® System available from Invitrogen. 6 Gateway®BP およびLR 組換え反応（続き） attB × attP 組換え反応の例 以下の図は、attB-PCR 産物とpDONR™ またはpDONR™/Zeo ベクターと の間のBP 組換え反応のスキームを示しています。この組換え反応によって、. **A second E. ColiExpression System is available with DH5!™ competent cells construction of Expression Clone but not for protein expression with pD, Life Technologies, a Division of Invitrogen Corporation www.doorway.ru Choosing Products to Build G GATEWAY Cloning Technology Instruction Manual, but does not.
specialized or customized vector, the Invitrogen™ Gateway™ Vector Conversion System can convert any vector into one compatible for Gateway cloning. Figure 1. Gateway technology facilitates cloning of genes into and back out of multiple vectors via site-specific recombination. Once a gene is cloned into an Entry clone, you can then. The easy-to-use choice for everyday cloning. Invitrogen Gateway recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. Life Technologies, a Division of Invitrogen Corporation www.doorway.ru Choosing Products to Build GATEWAY™ Expression Clones Step 1: Construct or Select an Entry Clone starting from: Design primers with attB sites Amplify PCR product Clone attB-PCR product into pDONR™ with BP CLONASE™ Enzyme Mix PCR Cloning System (BP CLONASE Mix.
The Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision recombination reactions that take. 9 sept Schematic modified from the Invitrogen MultiSite Gateway Pro user manual. Two-fragment Gateway MultiSite recombination cloning. A schematic. Perform a LR reaction between your entry clone and one of the pie-1 destination vectors (see protocol below). The resulting clone is ready for transformation.
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